Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100

Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871610LA01HU (8μg) + Jurkat whole cell lysate (500μg)
Lane 3: Jurkat whole cell lysate (10μg)